Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 8
Release date: Aug 14 2012
Last update date: Sep 3 2013
Access: Public
Taxon: Zea mays
Dataset link Effects of drought on gene expression in Maize reproductive and leaf meristem tissue revealed by RNA-seq

Experimental Protocol


Maize (Zea mays) plants of inbred line B73 were grown in the green house under well watered and drought stress conditions until they reached the reproductive stage (at the onset of silk emergence). For the drought stress two to three days after irrigation was withheld, the plants were hand pollinated, and 24 hours after pollination measurements and samples were collected for transcriptome analysis. At the end of the drought period (1DAP) the basal leaf meristem of the three youngest leaves and the ovary tissues were sampled for Illumina deep sequencing. Samples were labeled as well watered control leaf meristem (MLC), well watered control ovaries/ "cob" (MCC), drought stressed leaf meristem (MLD) and drought stressed ovary tissue (MCD). There are 8 libraries in total including one biological replicate for each condition.

Repositories


GEO

GSE40070

ArrayExpress

E-GEOD-40070

ENA

SRP014792

BioProject

PRJNA172724

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Contact


Akshay Kakumanu