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Specifications


Application: RNA-seq analysis
Number of samples: 24
Release date: Jul 31 2017
Last update date: Oct 30 2017
Access: Public
Dataset link Molecular signature for anastasis, recovery from the brink of apoptotic cell death

Experimental Protocol


3hr ethanol treatment was used to induce apoptosis in Hela cells. Ethanol was washed away after 3hr treatment to allow cells to recover. Total RNA was prepared from mock-treated cells, ethanol-treated cells and cells after 1hr, 2hr, 3hr, 4hr, 8hr, 12hr recovery, followed by ribosomal RNA depletion. 3 biological replicates were included for each group. Sequencing was done using Ion Proton.

Repositories


GEO

GSE86480

ENA

SRP086866

BioProject

PRJNA342034

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