Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 4
Release date: Feb 4 2013
Last update date: Nov 3 2017
Access: Public
Genes: DPP6
Taxon: Caenorhabditis elegans
Dataset link Identification of Transcription Factor DPL-1::GFP Binding Regions in L1

Experimental Protocol


EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: OP105(official name : OP105 genotype : unc119(ed3);wgIs105(dpl-1::TY1 EGFP FLAG;unc119) outcross : 0 mutagen : None tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for bombardment transformation of an unc-119(ed3) strain. The DPL-1::EGFP fusion protein has broad expression pattern description : but silence in germline cells. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the DPL-1 transcription factor. made_by : Bob Waterston's lab from UW ); Developmental Stage: fed L1; Genotype: unc119(ed3);wgIs105(dpl-1::TY1 EGFP FLAG;unc119); Sex: Hermaphrodite; EXPERIMENTAL FACTORS: Developmental Stage fed L1; Target gene dpl-1; Strain OP105(official name : OP105 genotype : unc119(ed3);wgIs105(dpl-1::TY1 EGFP FLAG;unc119) outcross : 0 mutagen : None tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for bombardment transformation of an unc-119(ed3) strain. The DPL-1::EGFP fusion protein has broad expression pattern description : but silence in germline cells. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the DPL-1 transcription factor. made_by : Bob Waterston's lab from UW ); temp (temperature) 20 degree celsius

Repositories


GEO

GSE44018

ArrayExpress

E-GEOD-44018

ENA

SRP018539

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DCC modENCODE