|Application:||Gene expression microarray analysis|
|Number of samples:||14|
|Release date:||Dec 25 2012|
|Last update date:||Aug 23 2018|
|Dataset link||Dynamic changes in the functions of Klf5 in maturing mouse corneas revealed by the changes in its target genes at postnatal days 11 and 56|
Wild type and Klf5-conditional null mouse corneal gene expression at postnatal day-11 and -56 was compared by Affymetrix mouse whole genome 430 2 arrays. Four age-matched PN56 WT and Klf5CN mice, and 3 age-matched PN11 WT and Klf5CN mice each were used for comparison of corneal gene expression by microarrays. Two dissected corneas from each mouse were pooled for isolation of total RNA using the RNeasy Mini kit (Qiagen, Germantown, MD). The quality and integrity of the isolated total RNA was confirmed using an Agilent Bioanalyzer. Total RNAs were amplified and labeled using a 3' IVT Express Kit (Affymetrix Inc., Santa Clara, CA), and hybridized to Affymetrix MG 430 2 chips following the protocol suggested by the manufacturer.
Shivalingappa Kottur Swamynathan
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