Computational protocol: Involvement of Three Esterase Genes from Panonychus citri (McGregor) in Fenpropathrin Resistance

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Protocol publication

[…] Besides PcE1, two novel CarE genes PcE7 and PcE9, were selected based on the analysis of our transcriptome data. The open reading frames of the genes were amplified, respectively, using the corresponding pair of specific primers () with the following procedure: 98 °C of initial incubation for 2 min followed by 35 cycles of 98 °C for 15 s, 60 °C for 15 s and 68 °C for 90 s; and 68 °C final extension for 10 min. The PCR products were purified from 1% agarose gel by MiniBEST Agarose Gel DNA Extraction Kit (Takara) and cloned into a pGEM-T Easy vector (Promega). Inserts were further sequenced for confirmation (BGI, Beijing, China). The sequences of CarEs from P. citri and T. urticae downloaded from the T. urticae genome portal website (http://bioinformatics.psb.ugent.be/orcae/overview/Tetur) were assembled by multiple sequence alignment using ClustalX []. Phylogenetic trees were constructed using a maximum likelihood method in MEGA 5.1, bootstrapping with 500 replicates []. […]

Pipeline specifications

Software tools Clustal W, MEGA
Databases Orcae
Application Phylogenetics
Organisms Panonychus citri, Spodoptera frugiperda
Diseases Drug-Related Side Effects and Adverse Reactions