Computational protocol: Protein array identification of protein markers for serodiagnosis of Mycobacterium tuberculosis infection

Similar protocols

Protocol publication

[…] All methods were carried out in accordance with the approved guidelines. The putative secreted and transmembrane proteins were predicted by bioinformatics tools, i.e. SignalP (http://www.cbs.dtu.dk/services/SignalP/) and TMHMM software (http://www.cbs.dtu.dk/services/TMHMM/), respectively. The RD proteins and the latent-associated proteins were selected from the relevant published data. All the selected genes were amplified by PCR and expressed in E. coli as GST fusion proteins (the details were included in ). […]

Pipeline specifications

Software tools SignalP, TMHMM
Application Membrane protein analysis
Organisms Mycobacterium tuberculosis, Homo sapiens
Diseases Tuberculosis
Chemicals Glutathione