Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 48
Release date: Aug 17 2016
Last update date: Nov 9 2017
Access: Public
Diseases: Leukemia
Dataset link Oncostatin M (OSM) and Leukemia Inhibitory factor (LIF) treatment of osteoblasts via LIF and OSM receptors

Experimental Protocol


Primary calvarial osteoblasts were generated from wild type and OSMR null mice, both on C57BL/6 background (cousin-bred litters), and frozen down for later treatment. On three occasions, WT and OSMR null cells were defrosted, expanded, and differentiated for 17 days in osteoblast differentiation media. At 17 days, when the cells have reached a stage of expressing osteocyte-specific genes, they were treated with human OSM (which acts only through LIFR in mouse cells), mouse LIF (which acts only through LIFR in mouse cells) or murine OSM (which can act through both OSMR and LIFR). RNA was analysed at 1 and 6 hours after commencement of treatment.

Repositories


GEO

GSE83418

BioProject

PRJNA325836

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Contact


Gordon Smyth