|Application:||Gene expression microarray analysis|
|Number of samples:||48|
|Release date:||Aug 17 2016|
|Last update date:||Nov 9 2017|
|Dataset link||Oncostatin M (OSM) and Leukemia Inhibitory factor (LIF) treatment of osteoblasts via LIF and OSM receptors|
Primary calvarial osteoblasts were generated from wild type and OSMR null mice, both on C57BL/6 background (cousin-bred litters), and frozen down for later treatment. On three occasions, WT and OSMR null cells were defrosted, expanded, and differentiated for 17 days in osteoblast differentiation media. At 17 days, when the cells have reached a stage of expressing osteocyte-specific genes, they were treated with human OSM (which acts only through LIFR in mouse cells), mouse LIF (which acts only through LIFR in mouse cells) or murine OSM (which can act through both OSMR and LIFR). RNA was analysed at 1 and 6 hours after commencement of treatment.