Computational protocol: Inhibition of breast cancer metastasis by co-transfection of miR-31/193b-mimics

Similar protocols

Protocol publication

[…] The mature miR-31-5p and miR-193b-3p sequences were obtained from miRBase database (http://www.mirbase.org). These oligomers were designed based on BLOCK-iT ™ Pol II miR RNAi Expression Vector Kits (Invitrogen). Then, the digested sequences were ligated into pcDNA 6.2gw/ EmGFP vector. To identify potential target genes of miR-193b and miR-31, the TargetScan (http://www.targetscan.org), miRanda (http://www.microrna.org), miRDB (http://www.mirdb.org/miRDB/) and PicTar (http://pictar.mdc-berlin.de) databases were used. […]

Pipeline specifications

Software tools TargetScan, MiRanda, PicTar
Databases microRNA.org miRDB miRBase
Application Non-coding RNA analysis
Diseases Breast Neoplasms