Computational protocol: Inhibition of breast cancer metastasis by co-transfection of miR-31/193b-mimics

Similar protocols

Protocol publication

[…] The mature miR-31-5p and miR-193b-3p sequences were obtained from miRBase database ( These oligomers were designed based on BLOCK-iT ™ Pol II miR RNAi Expression Vector Kits (Invitrogen). Then, the digested sequences were ligated into pcDNA 6.2gw/ EmGFP vector. To identify potential target genes of miR-193b and miR-31, the TargetScan (, miRanda (, miRDB ( and PicTar ( databases were used. […]

Pipeline specifications

Software tools TargetScan, MiRanda, PicTar
Databases miRDB miRBase
Application Non-coding RNA analysis
Diseases Breast Neoplasms