Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 23
Release date: Nov 3 2017
Last update date: Feb 2 2018
Access: Public
Dataset link Cold adaptation of tRNA nucleotidyltransferases: a tradeoff in activity, stability and fidelity

Experimental Protocol


in vivo tRNA from four Bacillales species was isolated from exponential and stationary growth phase each in triplicates resulting in a total of 24 samples. Sequencing libraries were created for small RNA species and sequenced on 3 runs of a MiSeq using 8 indices. Reads were analyzed regarding 3'-ends of tRNA and results summed up as a mean for the 8 conditions investigated. Results from B. subtilis were regarded as reference sample for a mesophilic Bacillales species. Differences towards psychophilic Planococcus halocryophilus and Exiguobacterium sibiricum and thermophilic Geobacills stearothermophilus were investigated.

Repositories


GEO

GSE98874

ENA

SRP106999

BioProject

PRJNA386487

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