Computational protocol: Characterization of a novel N-acetylneuraminic acid lyase favoring N-acetylneuraminic acid synthesis

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Protocol publication

[…] Basic Local Alignment Search Tool (BLAST) of NCBI was used to identify proteins homologous to CgNal, and MEGA 5.2 was used to construct the phylogenetic tree with bootstrap value calculated after 1000 generations. CgNal subunit structure was modeled by Phyre2 server at intensive mode. Sequences were aligned with ClustalW and ESPript. […]

Pipeline specifications

Software tools BLASTN, MEGA, Phyre, Clustal W, ESPript
Application Phylogenetics
Organisms Corynebacterium glutamicum ATCC 13032
Chemicals N-Acetylneuraminic Acid, Pyruvic Acid