Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Nov 9 2011
Last update date: Jul 6 2016
Access: Public
Chemicals: Purines, Tryptophan
Dataset link Mixed Culture Gene Expression of E. coli and Pseudomonas aeruginosa

Experimental Protocol


Two replicates of LB grown E. coli ZK126 and P. aeruginosa PAO1 were grown in pure culture at 37C to an OD of 0.3 (log phase) and then mixed 1:1 and incubated for an additional 45 min. RNA was extracted, purified, reverse transcribed to cDNA and then analyzed on E. coli and P. aeruginosa chips from Affymetrix. Expression profiles were compared to pure culture E. coli and P. aeruginosa grown on LB.

Repositories


GEO

GSE26931

ArrayExpress

E-GEOD-26931

BioProject

PRJNA136083

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Contact


Robert McLean
Robert JC McLean