Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 96
Release date: May 29 2018
Last update date: Nov 20 2018
Access: Public
Diseases: Disease Models, Animal, Epididymitis, Hypothalamic Diseases, Hypothalamic Neoplasms, Neoplasms, Adipose Tissue
Chemicals: Sucrose
Genes: AGRP, TGFB1, PDK4, FGF1
Dataset link Dietary fat, but not dietary protein or carbohydrate (sucrose), regulates energy intake and causes adiposity in mice

Experimental Protocol


In total 96 samples are analyzed. From each diet group, the hypothalami of 8/20 individuals were collected. The left halves of two, and the right halves of another two, were pooled together as one sample, and the same was performed with the other 4 hypothalami, resulting in each diet group having 2 pooled samples of 4 hypothalami (n = 48 samples in total across 24 diets). From each diet group, the sWAT and eWAT of 12/20 individuals were also collected. A small piece from each of six sWAT collections were pooled together as one sample, and the same was performed with the other six eWAT collections. In this way each diet group had one pooled sWAT sample and one pooled eWAT sample (also n = 48 across 24 diets)

Repositories


GEO

GSE114960

ENA

SRP149081

BioProject

PRJNA473341

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Contact


John Speakman