Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 14
Release date: Jan 21 2016
Last update date: Jun 28 2018
Access: Public
Diseases: Neoplasms, Thyroid Neoplasms
Dataset link Genomic binding and regulation of gene expression by the thyroid carcinoma-associated PAX8-PPARG fusion protein

Experimental Protocol


The rat PCCL3 thyroid cell line was stably transfected with human PAX8-PPARG fusion protein (PPFP, myc-tagged) or empty vector. For RNA-Seq, cells were cultured ±1 μM pioglitazone for 16 hours before RNA isolation, and 3 independent experiments were performed. For ChIP-seq, cells were cultured with 1 μM pioglitazone for 16 hours and IP was performed with anti-myc tag antibody.

Repositories


GEO

GSE70354

ENA

SRP059939

BioProject

PRJNA288400

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Contact


Bing Ren