Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Nov 21 2007
Last update date: Aug 28 2018
Access: Public
Dataset link DELLA protein direct targets in Arabidopsis

Experimental Protocol


Seedlings of both transgenic lines were pretreated for 16 h with 2 uM GA4 to enhance gibberellin responses. Because DELLA proteins are strong signaling repressors, this pretreatment should maximize the effect of DELLA induction. Eight-day old seedlings were treated with 2 uM GA4 or a combination of 2 uM GA4 plus 10 uM DEX to induce the rga-delta17 transgene. Three biological replicas for the transgenic line that carries the DEX-inducible rga-delta17 transgene were generated at 2h and 4h. For the empty vector line, only 2 biological replicas were generated at 4h of treatment with 2 uM GA with or without 10 uM DEX.

Repositories


GEO

GSE8741

ArrayExpress

E-GEOD-8741

BioProject

PRJNA105285

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Contact


Rodolfo Zentella