Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Oct 16 2014
Last update date: Oct 16 2014
Access: Public
Taxon: Plasmodium falciparum
Dataset link Identification of a subtelomeric gene family expressed during the asexual-sexual stage transition in Plasmodium falciparum

Experimental Protocol


Microarray analysis was used to compare two clones derived from Plasmodium falciparum strain 3D7 parasites that differ in their ability to undergo gametocytogenesis. Clone G+ produces gametocytes and clone G- produces very few if any gametocytes. RNA was harvested from the cultures when the asexual parasitemia was 0.9-1.48% (day 4) (n=4) after setting up the gametocyte cultures and 5.2-5.58% (day 6) (n=4) prior to the appearance of morphologically distinct gametocytes and used to generate cDNA that was labeled with Cy3 or Cy5 and hybridized to the Plasmodium falciparum 70 mer oligonucleotide microarray developed by DeRisi and co-workers.

Repositories


GEO

GSE62364

ArrayExpress

E-GEOD-62364

BioProject

PRJNA263917

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Contact


Tetsuya Furuya

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