Computational protocol: CD3brightCD56+ T cells associate with pegylated interferon-alpha treatment nonresponse in chronic hepatitis B patients

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Protocol publication

[…] After removing plasma from blood samples, the indicated fluorochrome-conjugated monoclonal antibodies were added to 100 μL whole blood. The red blood cells were lysed with RBC lysis buffer using standard procedures. Stained cells were collected using an LSR II flow cytometer (BD Biosciences), and data were analyzed using FlowJo analysis software 7.6.1 (Tree Star, Inc.). Human CD56+ T cells were gated as CD3+ CD56+ lymphocytes for further analysis. [...] A two-tailed Student’s t-test was used to statistically compare differences between two groups. A two-way ANOVA test was used to statistically compare differences among multiple groups at different time points after treatment. Data are expressed as mean ± standard error of the mean (s.e.m.). Statistical analyses were performed with Prism 6 (GraphPad Software, Inc.) or SPSS Statistics version 22 (IBM). Differences achieving values of P < 0.05 were considered to be statistically significant. […]

Pipeline specifications

Software tools FlowJo, SPSS
Applications Miscellaneous, Flow cytometry
Organisms Homo sapiens
Diseases Hepatitis B, Hepatitis B, Chronic
Chemicals Nucleosides, Nucleotides