Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 40
Release date: Oct 26 2009
Last update date: Mar 21 2012
Access: Public
Diseases: Infection, Mastitis
Chemicals: Amino Acids
Dataset link The effect of energy balance on bovine mammary tissue gene expression during Streptococcus uberis mastitis challenge

Experimental Protocol


10 Holstein cows in mid-lactation (~90 days in milk) were subjected to either dietary-induced negative energy balance (n=5) or fed ad libitum to maintain positive energy balance (n=5). The cows were inoculated with 5,000 cfu of Streptococcus uberis strain O140J into the mammary quarter. Both the infected and non-infected rear quarters of all cows were biopsied at 20 hours post-inoculation for gene expression profiling. Mammary tissue was cohybridzed with RNA from a pool of bovine tissues. All samples were hybridized to duplicate slides with reverse labeling (40 microarrays total). The supplementary file 'GSE15344_raw_data_readme.txt' contains a description of the contents of the raw data files.

Repositories


GEO

GSE15344

BioProject

PRJNA116573

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Contact


Kasey Moyes

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