Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 9
Release date: Aug 21 2012
Last update date: Jan 23 2018
Access: Public
Diseases: Cluster Headache, Leukemia, Myeloid, Myeloproliferative Disorders, Neural Tube Defects, Leukemia, Myeloid, Acute
Genes: ASXL1
Dataset link ASXL1 Mutations Promote Myeloid Transformation Through Loss of PRC2-Mediated Gene Repression

Experimental Protocol


To assess the genome-wide effects of ASXL1 loss on chromatin state we performed chromatin immunoprecipitation followed by next generation sequencing (CHIP-seq) for histone modifications known to be associated with PcG (histone H3 lysine 27 trimethylation (H3K27me3)) or TxG activity (histone H3 lysine 4 trimethylation (H3K4me3)) in UKE1 cells expressing empty vector (EV) or 2 independent validated shRNAs for ASXL1. This Series represents the ChIP-Seq data (not the microarray data referenced in the summary above). The related micorarray data are available in GEO as GSE38692.

Repositories


GEO

GSE38861

ArrayExpress

E-GEOD-38861

ENA

SRP013855

BioProject

PRJNA169078

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