Dataset features


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Oct 19 2009
Last update date: Jul 6 2016
Access: Public
Diseases: DNA Repair-Deficiency Disorders
Chemicals: Nucleotides
Dataset link Transcriptomic response of young adult N2, xpa1, and glp1 nematodes 3h after exposure to 50 J/m2 UVC radiation at 25°C

Experimental Protocol

Young adult (24 h post-vulval crescent L4 stage) N2, xpa-1, and glp-1 nematodes were reared at 25°C and exposed to 50 J/m2 UVC radiation on K-agar plates without food as described (Meyer et al., 2007 Genome Biology 8(5):R70), placed on OP50-seeded plates for 2 h 45 minutes, washed off plates and rinsed with K-medium 3 times (~5 minutes each) by allowing settling, resuspending, settling again, etc. Nematodes were frozen and RNA extracted. The experiment was performed twice, once in 2007 and once in 2008, with 2 replicates each time, for a total “n” of 4 per strain per treatment (control vs UVC-exposed).








NIEHS Microarray Core