Computational protocol: One of Three Pex11 Family Members Is Required for Peroxisomal Proliferation and Full Virulence of the Rice Blast Fungus Magnaporthe oryzae

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Protocol publication

[…] The Pex11 homologues were identified by searching from the Genbank or the fungal genome database (http://www.broadinstitute.org/scientific-community/data). The real coding sequences of MoPEX11A, MoPEX11B and MoPEX11C were determined by PCR amplification using the cDNA revers-transcripted from the total RNA of the fungus as template. Sequence alignments were performed using the Clustal W method, and imported into the software GeneDoc 2.0 (http://genedoc.software.informer.com/download/) for type setting and into MEGA version 5.0 (http://www.megasoftware.net/) to establish the phylogenetic trees. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Magnaporthe oryzae