Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 36
Release date: Apr 24 2008
Last update date: Aug 28 2018
Access: Public
Dataset link Expression analysis of root cell-types after treatment with salt

Experimental Protocol


To gain a genome-scale understanding of the role that developmental processes play in regulating stimulus response, we examined the effect of salt stress on gene expression along the radial axis of the root. Cell identity is the main variable that changes along the radial axis with the epidermis representing the outermost tissue layer and the stele representing the inner most layer. 6 different GFP reporter lines were used to isolate specific populations of cells from the Arabidopsis root using FACS sorting of protoplasted cells. GFP-reporter lines were grown under standard conditions before protoplasting and sorting or they were transfered to media supplemented with 140mM NaCl for 1 hour before hand.

Repositories


GEO

GSE7641

ArrayExpress

E-GEOD-7641

BioProject

PRJNA99837

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Contact


Jose Dinneny
Jose Ramon Dinneny