|Application:||Gene expression microarray analysis|
|Number of samples:||36|
|Release date:||Apr 24 2008|
|Last update date:||Aug 28 2018|
|Dataset link||Expression analysis of root cell-types after treatment with salt|
To gain a genome-scale understanding of the role that developmental processes play in regulating stimulus response, we examined the effect of salt stress on gene expression along the radial axis of the root. Cell identity is the main variable that changes along the radial axis with the epidermis representing the outermost tissue layer and the stele representing the inner most layer. 6 different GFP reporter lines were used to isolate specific populations of cells from the Arabidopsis root using FACS sorting of protoplasted cells. GFP-reporter lines were grown under standard conditions before protoplasting and sorting or they were transfered to media supplemented with 140mM NaCl for 1 hour before hand.
Jose Ramon Dinneny