
Similar protocols
Protocol publication
[…] Human cytomegalovirus gO (UL74 gene) genotyping was performed on a subset of 34 milk samples with sufficient remaining volume. Based on translated amino acid sequences, HCMV gO (UL74 gene) has 8 distinct genotypes: gO1a, gO1b, gO1c, gO2a, gO2b, gO3, gO4, and gO5 [, ]. We used PCR-based genotyping with the gOup/gOlw primers, which detected all 8 gO genotypes. Nucleotide sequences were determined using Sanger methods [, ] and compiled using ChromasPro software. Multiple alignments used CLUSTAL and phylogenetic analysis via maximum-likelihood methods in MEGA6 []. […]
Pipeline specifications
Software tools | ChromasPro, MEGA |
---|---|
Application | Phylogenetics |
Organisms | Homo sapiens, Human immunodeficiency virus 2, Human betaherpesvirus 5 |
Diseases | Cytomegalovirus Infections, HIV Infections |