Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 9
Release date: Oct 1 2008
Last update date: Aug 10 2018
Access: Public
Diseases: Neoplasms
Dataset link EZH2 is a mediator of EWS-FLI1 driven tumor growth blocking endothelial and neuro-ectodermal differentiation

Experimental Protocol


A673 cells were treated for 24 hours either with 100 nM Trichostatin A (TSA) or 0.01% DMSO. In siRNA experiments with ezh2 specific siRNA A673 cells were resuspended in medium containing 5 nM siRNA and transfection reagent and incubated for 48 hours. RNA from cells under such treatment was isolated with Trizol and subjected to microarray analysis onto human U133A microarray following the Affymetrix protocol.

Repositories


GEO

GSE12692

ArrayExpress

E-GEOD-12692

BioProject

PRJNA112701

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Contact


Guenther Richter