Microbiota-derived short-chain fatty acids modulate expression of Campylobacter jejuni determinants required for commensalism and virulence

Two-condition experiment, WT C. jejuni 81-176 SmR (DRH212) and isogenic ΔCjjptaackA mutant. Biological replicates: 3 81-176 SmR and 3ΔCjjptaackA independently grown to log phase and harvested. Two technical replicates/biological replicate. One replicate per array. A type 2 gene expression experimental design was used, with fluorescently labeled genomic DNA as a reference channel in each experiment as described by Lucchini, S., et al. 2005. Infect Immun 73:88-102.