Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Sep 2 2016
Last update date: Sep 4 2016
Access: Public
Diseases: Dysentery
Chemicals: Amino Acids, Butyrates, Fatty Acids, Fatty Acids, Volatile, Lactic Acid
Dataset link Microbiota-derived short-chain fatty acids modulate expression of Campylobacter jejuni determinants required for commensalism and virulence

Experimental Protocol


Two-condition experiment, WT C. jejuni 81-176 SmR (DRH212) and isogenic ΔCjjptaackA mutant. Biological replicates: 3 81-176 SmR and 3ΔCjjptaackA independently grown to log phase and harvested. Two technical replicates/biological replicate. One replicate per array. A type 2 gene expression experimental design was used, with fluorescently labeled genomic DNA as a reference channel in each experiment as described by Lucchini, S., et al. 2005. Infect Immun 73:88-102.

Repositories


GEO

GSE86327

BioProject

PRJNA341455

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Contact


Craig Parker