Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 33
Release date: Nov 10 2008
Last update date: Apr 5 2017
Access: Public
Taxon: Equus caballus
Dataset link Expression data from PBMC treated with rabbit anti-thymocyte globulin (rATG) or horse ATG (hATG)

Experimental Protocol


PBMC from healthy controls were cultured with rATG or hATG (each at 10 ug/mL) in 10% FCS/RPMI 1640 at 37C for 24 hours, PBMC without drugs were used as controls. Total RNA was extracted with the RNeasy kit (Qiagen, Valencia, CA). RNA purity was assessed by spectrophotometry. Probes were prepared using standard Affymetrix protocols and hybridized to Affymetrix HG-U133A 2.0 arrays (Affymetrix, Santa Clara, CA)

Repositories


GEO

GSE10040

ArrayExpress

E-GEOD-10040

BioProject

PRJNA108607

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Contact


Xingmin Feng