|Dataset type:||Expression profiling by high throughput sequencing|
|Number of samples:||6|
|Release date:||Jan 1 2019|
|Last update date:||Feb 16 2019|
|Dataset link||Reversing Abnormal Neural Development by Inhibiting OLIG2 in Down Syndrome Human iPSC Brain Organoids and Neuronal Mouse Chimeras|
Organoids (5-week old) generated from hiPSC lines derived from two DS patients (patient DS1 and DS3 and corresponding cell lines include DS1, control1, isogenic Tri-DS3 and Di-DS3, Tri-DS3+ContshRNA, and Tri-DS3+OLIG2shRNA hiPSCs) were used for RNA-sequencing. For each cell line, total 40-60 organoids collected from three batches of organoid cultures were pool together for RNA extraction and sample preparation. By pooling organoids, we increased the chances of identifying genes that were commonly up- or down-regulated in the three batches of cultures, whereas on the other hand, we decreased the chances of identifying genes that had varied expression levels in the three batches of cultures.