Dataset features

Specifications


Dataset type: Expression profiling by high throughput sequencing
Number of samples: 6
Release date: Jan 1 2019
Last update date: Feb 16 2019
Access: Public
Dataset link Reversing Abnormal Neural Development by Inhibiting OLIG2 in Down Syndrome Human iPSC Brain Organoids and Neuronal Mouse Chimeras

Experimental Protocol


Organoids (5-week old) generated from hiPSC lines derived from two DS patients (patient DS1 and DS3 and corresponding cell lines include DS1, control1, isogenic Tri-DS3 and Di-DS3, Tri-DS3+ContshRNA, and Tri-DS3+OLIG2shRNA hiPSCs) were used for RNA-sequencing. For each cell line, total 40-60 organoids collected from three batches of organoid cultures were pool together for RNA extraction and sample preparation. By pooling organoids, we increased the chances of identifying genes that were commonly up- or down-regulated in the three batches of cultures, whereas on the other hand, we decreased the chances of identifying genes that had varied expression levels in the three batches of cultures.

Repositories


GEO

GSE124513

ENA

SRP174927

BioProject

PRJNA512272

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Contact


Ying Liu