Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Sep 24 2006
Last update date: Jan 27 2016
Access: Public
Chemicals: Zinc
Dataset link Profiling of zinc altered gene expression in human prostate normal (HPR-1) and malignant (PC-3) cells

Experimental Protocol


Zinc effect on gene expression of HPR-1 and PC-3 cells was evaluated with a time-course study using human genomewise microarray chips (Applied Biosystems v 2.0). For each chip, 2µg total RNA from each sample was used. A series of samples from each cell line was prepared as follows: control, 1 hr, 3 hrs, and 6 hrs zinc exposure. Synthesis of cRNA and subsequent hybridization was completed by the Core Facility at UMBI according to the ABI standard protocol. The ABI human genome survey microarray chip contained 32,878 probes for the interrogation of 29,098 known genes

Repositories


GEO

GSE5590

BioProject

PRJNA96879

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Contact


Shufei Lin