Computational protocol: Human A53T α-Synuclein Causes Reversible Deficits in Mitochondrial Function and Dynamics in Primary Mouse Cortical Neurons

Similar protocols

Protocol publication

[…] The spinning disk confocal microscope consisted of a Nikon Eclipse Ti (Nikon) base with spinning disk CSU-X1 (Yokogawa), Nikon 100X/1.40NA, Nikon 60X/1.40NA or Nikon 40X/1.30NA objectives, a Nikon perfect focus system for continuous automatic focusing of the sample during time-lapse imaging and an EMCCD camera (Andor iXON X3). During time-lapse microscopy neurons were kept at 37 °C within an incubation chamber (Okolab). All images were collected, measured and compiled in ImageJ (NIH) or MetaMorph imaging software (Molecular Devices). Figures were compiled in Illustrator (Adobe). A Zeiss LSM510 confocal microscope was also used to acquire some time-lapse images at the beginning of the project. […]

Pipeline specifications

Software tools ImageJ, MetaMorph
Applications Laser scanning microscopy, Microscopic phenotype analysis
Organisms Mus musculus, Homo sapiens
Diseases Movement Disorders, Parkinson Disease, Neurodegenerative Diseases, Mitochondrial Diseases, Drug-Related Side Effects and Adverse Reactions