Computational protocol: Hepatitis E Virus Superinfection and Clinical Progression in Hepatitis B Patients

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Protocol publication

[…] PCR products were purified (Exo-SAP-IT kit; USB, Affymetrix, USA) and used as sequencing templates (BigDye terminator v.1.1 sequencing kit; Applied Biosystems, USA, ABI 3130XL sequencer). HEV genotyping was performed by phylogenetic analyses based on the amplified ORF2 sequences using the MEGA 5 software (www.megasoftware.net). HEV reference sequences were obtained from the NCBI GenBank database. [...] Analyses were performed using the SPSS software (SPSS Statistics, IBM, Armonk, NY) and Intercooled Stata (Stata Corporation, College Station, TX, USA). Prevalences and quantitative variables are given as percentages and medians with ranges. Categorical variables were compared applying Chi square or Fisher's exact tests. Kruskal–Wallis or Mann–Whitney U tests were used to compare quantitative variables. A logistic regression model was used to compare HEV seroprevalences between groups and to analyze associations of HEV seroprevalences with disease progression. Odds ratios (OR) and 95% confidence intervals (CI) were calculated. The level of significance was P < 0.05. […]

Pipeline specifications

Software tools MEGA, SPSS
Applications Miscellaneous, Phylogenetics
Organisms Hepatitis E virus, Hepatitis B virus, Homo sapiens
Diseases Hepatitis B, Carcinoma, Hepatocellular, Liver Cirrhosis, Liver Diseases, Liver Failure, Chemical and Drug Induced Liver Injury