|Number of samples:||15|
|Release date:||May 31 2018|
|Last update date:||Nov 19 2018|
|Diseases:||Brain Neoplasms, Glioblastoma, Neoplasms|
|Dataset link||The transition from proliferation to quiescence in glioblastoma stem-like cells requires Ca2+ signaling and mitochondria remodeling|
To establish RNA signatures of proliferative and quiescent GSLCs we have adopted the following strategy. Several experiments were performed in order to take into account different type of variabilities (Table S1); (1) Variability due to laboratories environments; experiments have been done in two laboratories ( Strasbourg and Toulouse) following identical protocols. The cells were obtained from the same master cell bank and the composition of the growing media was identical. (2) Cellular variability: two cell lines TG1 and OB1 were used. (3) Variability in inducing quiescence; the switch to quiescent state was obtained by either the non replacement of the medium during 9 days, acidification of the medium to pH 6.5 or 6.2 for 5 days or treatment of the cell by 10 µM SKF-96365 in the growing medium (pH 7.4) at day 1 and 3 and analysis at day 5. In normal medium at pH 7.4, the cells are in a proliferative state. Total RNA was extracted as described above and RNA quality was controlled with AATI Fragment Analyser (Advanced Analytical Technologies, Inc). RNA-seq was obtained from the IGBMC platform and the short reads were aligned using the reference genome hg38 (http://genomeast.igbmc.fr). The Qlucore software (http://www.qlucore.org) was used for the analysis.