|Application:||Gene expression microarray analysis|
|Number of samples:||78|
|Release date:||May 11 2012|
|Last update date:||Dec 22 2017|
|Diseases:||Tongue Diseases, Tongue Neoplasms, Tumor Lysis Syndrome|
|Dataset link||Gene expression profiling of archival tongue carcinoma and normal tongue tissue (all samples)|
Total RNA from formalin fixed paraffin embedded sample was extracted using the High Pure RNA Paraffin Kit according to the manufacturer’s protocol. Quality of FFPE sample were evaluated by comparing how well a kousekeeping gene (TUBA6) could be amplified using q-PCR in each FFPE sample compared to two fresh frozen sample (Ctdiff= CTffpe-Ctff). The DASL platform (cDNA-mediated annealing, selection, extension, and ligation assay) was used together with the whole genome arrays to obtain expression data for 20 818 genes.
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