Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 78
Release date: May 11 2012
Last update date: Dec 22 2017
Access: Public
Diseases: Tongue Diseases, Tongue Neoplasms, Tumor Lysis Syndrome
Chemicals: Formaldehyde
Dataset link Gene expression profiling of archival tongue carcinoma and normal tongue tissue (all samples)

Experimental Protocol


Total RNA from formalin fixed paraffin embedded sample was extracted using the High Pure RNA Paraffin Kit according to the manufacturer’s protocol. Quality of FFPE sample were evaluated by comparing how well a kousekeeping gene (TUBA6) could be amplified using q-PCR in each FFPE sample compared to two fresh frozen sample (Ctdiff= CTffpe-Ctff). The DASL platform (cDNA-mediated annealing, selection, extension, and ligation assay) was used together with the whole genome arrays to obtain expression data for 20 818 genes.

Repositories


GEO

GSE34105

ArrayExpress

E-GEOD-34105

BioProject

PRJNA156487

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Contact


Matilda Rentoft

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