Computational protocol: Transition-state destabilization reveals how human DNA polymerase β proceeds across the chemically unstable lesion N7-methylguanine

Similar protocols

Protocol publication

[…] The binary and ternary polβ complexes with templating Fm7dG were crystallized using the similar conditions described previously (). Diffraction data were collected at 100 K at the beamline 5.0.3 at the Advanced Light Source, Lawrence Berkeley National Laboratory. All diffraction data were processed using HKL2000. Structures were solved by molecular replacement using a gapped binary complex structure with an open conformation (PDB ID: 1BPX) and a ternary complex structure with a closed conformation (PDB ID: 1BPY) as the search models. The model was built using Coot and refined using Phenix. […]

Pipeline specifications

Software tools Coot, PHENIX
Application Protein structure analysis
Organisms Homo sapiens, Dipturus trachyderma
Diseases Neurofibromatosis 1