Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 16
Release date: Sep 15 2015
Last update date: Oct 24 2018
Access: Public
Diseases: Bacterial Infections, Colitis, Ulcerative, Colonic Diseases, Mucositis
Chemicals: Amino Acids, Gentamicins
Dataset link RNA-sequencing of mRNAs from control and CAP-D3 deficient Salmonella infected HT-29 cells

Experimental Protocol


Three RNA samples from 3 independent experiments including timepoints taken at 0, 0.5 and 7 hours post-infection were analyzed on a bioanalyzer for quality; one of the 0.5 hour post-infection samples was excluded at this time due to poor RNA purity. Directional, cDNA libraries made from cellular mRNAs were generated from the other 8 samples and sequenced (paired-end sequencing of 100 bp reads) in the Genomics Core at the University of Chicago on an Illumina HiSeq2000.

Repositories


GEO

GSE62520

ArrayExpress

E-GEOD-62520

ENA

SRP049063

BioProject

PRJNA264328

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Contact


Michelle Longworth
Michelle Suzanne Longworth