Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 30
Release date: Dec 11 2012
Last update date: Sep 13 2014
Access: Public
Diseases: Bone Diseases, Joint Diseases, Osteoarthritis
Dataset link Gene Expression Analyses of Subchondral Bone in Early Experimental Osteoarthritis by Microarray

Experimental Protocol


Ninety 10-week-old male Sprague-Dawley rats, weighing 300-325g, were used in the study. Animals were equally divided into two groups: experimental group (E-Group) and sham-operated group (S-Group). The E-Group rats underwent open surgery, involved in both medial meniscectomy and medial collateral ligament (MCL) transaction with micro-scissors. The S-Group rats were carried out with a sham operation, via a similar incision, without operations of the medial meniscus and the medial collateral ligament.Animals were killed at 1, 2, and 4 weeks postsurgery, and 15 animals were put into use per-timepoint in each treatment group. 5 animals were used for histological analysis and immunohistochemistry, and others were used for microarray study and Real-time polymerase chain reaction (PCR) analysis equally at each timepoint.

Repositories


GEO

GSE30322

ArrayExpress

E-GEOD-30322

BioProject

PRJNA143609

Download


Contact


DaoZhang Cai