Dataset features

Specifications


Application: DNA methylation array analysis
Number of samples: 31
Release date: Dec 20 2011
Last update date: Jan 2 2015
Access: Public
Diseases: Adenoma, Islet Cell, Ovarian Diseases, Ovarian Neoplasms
Chemicals: Cisplatin
Dataset link Genome-wide Methylation Profiling Identifies Candidate DNA Methylation Drivers of Acquired Cisplatin Resistance in Ovarian Cancer.

Experimental Protocol


Array-based methylation profiling was performed using the Infinium HumanMethylation27 BeadChip in two cisplatin sensitive cell lines and three cisplatin resistant cell lines derived in vitro, four pairs of cisplatin sensitive and resistant cell lines derived in vivo, 7 pairs of tumour tissues obtained from patients before chemotherapy and at disease relapse, 2 pairs of IGROV1 SP and NSP cells. The reproducibility of the Infinium HumanMethylation27 BeadChips was evaluated using biological and technical replicates of matched chemosensitive/chemoresistant ovarian cancer cell lines PEO1/PEO4. Differential methylation cutoff was estimated from two biological replicates by bootstrap resampling.

Repositories


GEO

GSE28647

ArrayExpress

E-GEOD-28647

BioProject

PRJNA143077

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Contact


Wei Dai