Computational protocol: Oxidative Stress Resistance in Metastatic Prostate Cancer: Renewal by Self-Eating

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Protocol publication

[…] Quantitative phase imaging is a non-invasive technique with high intrinsic contrast even for naturally transparent objects such as live cells. Therefore this method is suitable for long term observations of cell reactions to treatment without any additional staining. In these experiments quantitative phase imaging was performed by Tescan multimodal holographic microscope Q-PHASE. Q-PHASE is based on the original concept of coherence-controlled holographic microscope [, ].Quantitative phase imaging was initiated immediately after plumbagin treatment. Cells were cultivated in Flow chambers μ-Slide I Lauer Family (Ibidi, Martinsried, Germany) In order to image enough number of cells in one field of view, objectives Nikon Plan 10/0.30 were chosen. Holograms were captured by CCD camera (XIMEA MR4021 MC-VELETA). The entire image reconstruction and image processing were performed in Q-PHASE control software. Quantitative phase images are shown in grayscale with units of pg/μm2 that were recalculated from original radians according to Barer and Davies [, ]. Movies with identification arrows were prepared in ImageJ software. [...] Pearson correlation, principal component analysis and cluster analysis were performed to reveal associations between cases and variables. These analyses were performed on standardized data; the cluster analysis was performed using Ward’s method. All charts are depicted with means and standard deviations. Software Statistica (StatSoft, Tulsa, OK, USA) was used for analysis. […]

Pipeline specifications

Software tools ImageJ, Statistica
Applications Miscellaneous, Microscopic phenotype analysis
Diseases Neoplasms, Prostatic Neoplasms