Dataset features


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Jan 24 2017
Last update date: Jan 26 2017
Access: Public
Dataset link Expression data from additional molar germs at stages of early development in miniature pigs

Experimental Protocol

Miniature pigs' additional molar germs samples from early developmental stages at E50, E60 and E70 were selected for RNA extraction and hybridization on Affymetrix microarrays in biological triplicates. The differentially expressed genes between the 3 different stages were screened, then differentially expressed genes with significance were chosen for significant GO and pathway enriching. The differential genes and functions with significant expression tendencies were predicted using STC for further Path-Net, Signal-Net and Dynamic-Gene-Net analysis for Signal-Net to determine significant pathways and identify the key differentially expressed genes during early additional molars development. Additional molar germs from three key stages were chosen to determine the spatiotemporal control mechanisms of cascade initiation of these molars. The E50 stage corresponding to M1 at cap stage without M2 initiation and the E60 stage targeting M1 at bell stage followed by M2 initiation. The E70 stage as a control, when M1 progresses to late bell stage but M2 changes little.