Dataset features

Specifications


Application: ChIP-seq analysis, RNA-seq analysis
Number of samples: 34
Release date: Jun 13 2014
Last update date: Oct 24 2018
Access: Public
Taxon: Mus musculus
Dataset link piRNA pathway targets active LINE1 elements to establish repressive H3K9me3 mark in germ cells

Experimental Protocol


Total of 34 libraries were analyzed. In case of ChIP libraries, every 'input' sample was used for normalization of the respective ChIP (H3K9me3 or H3) sample; the input libraries preceed ChIP libraries in the list below. There are four replicates (input-ChIP pairs) for H3K9me3 ChIP on liver cells, two for ChIP on testicular somatic cells for each genotype (Miwi2 Het and KO); and four for ChIP on male germ cells for each genotype (one from FACS-sorted germ cells, and three from MACS-sorted germ cells). Each replicate has a Het/KO pair, and the respective libraries were cloned from the material isolated from littermates. One H3 ChIP for each genotype is included, with respective inputs.

Repositories


GEO

GSE58332

ArrayExpress

E-GEOD-58332

ENA

SRP043149

BioProject

PRJNA252565

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Contact


Sergei Manakov
Sergei A Manakov