Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Jun 28 2007
Last update date: Jul 1 2016
Access: Public
Diseases: Inflammatory Bowel Diseases
Chemicals: Bile Acids and Salts, Triglycerides
Dataset link Genome-wide analysis of PPARα activation in murine small intestine

Experimental Protocol


Pure bred wild-type (129S1/SvImJ) and PPARα-null (129S4/SvJae) mice were treated with the synthetic PPARα ligand WY14,643 (0.1% w/w) for 5 days. The complete intestines were then removed and total RNA was isolated. In total 4 experimental groups were present: wild type mice fed the control diet (AIN93M), wild type mice fed the control diet supplemented with 0.1% w/w WY14,643 for 5 days, PPARα knockout mice fed the control diet, PPARα knockout mice fed the control diet supplemented with 0.1% w/wWY14,643 for 5 days. RNA of 3 biological replicates was hybridized to Affymetrix 430A arrays. Five microgram total RNA was labelled according to the ENZO-protocol, fragmented and hybridized according to Affymetrix's protocols.

Repositories


GEO

GSE5475

ArrayExpress

E-GEOD-5475

BioProject

PRJNA95989

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Contact


Guido Hooiveld