|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Jun 28 2007|
|Last update date:||Jul 1 2016|
|Diseases:||Inflammatory Bowel Diseases|
|Chemicals:||Bile Acids and Salts, Triglycerides|
|Dataset link||Genome-wide analysis of PPARα activation in murine small intestine|
Pure bred wild-type (129S1/SvImJ) and PPARα-null (129S4/SvJae) mice were treated with the synthetic PPARα ligand WY14,643 (0.1% w/w) for 5 days. The complete intestines were then removed and total RNA was isolated. In total 4 experimental groups were present: wild type mice fed the control diet (AIN93M), wild type mice fed the control diet supplemented with 0.1% w/w WY14,643 for 5 days, PPARα knockout mice fed the control diet, PPARα knockout mice fed the control diet supplemented with 0.1% w/wWY14,643 for 5 days. RNA of 3 biological replicates was hybridized to Affymetrix 430A arrays. Five microgram total RNA was labelled according to the ENZO-protocol, fragmented and hybridized according to Affymetrix's protocols.