Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Feb 15 2012
Last update date: May 3 2013
Access: Public
Taxon: Homo sapiens
Dataset link Maternal tract distinguish between spermatozoa and other pathogenic and non-pathogenic entities

Experimental Protocol


Oviductal epithelial cells (OPEC) were co-incubated in the presence of (i) bacteria (1 x 10˄8 bacteria), (ii) sperm (1 X 10 ˄ 6 spermatozoa/ml) or (iii) red blood cell (5 x 10 ˄ 6 red blood cell/ml) for 24 hours in HAM-12 media. Three biological replicates were performed for each group and a total of 12 Affymetrix Porcine arrays were used to identify the expression profiles of OPEC alone (3 arrays) or in the presence of (i) bacteria (3 arrays), (ii) sperm (3 arrays) or (iii) red blood cells (3 arrays).

Repositories


GEO

GSE34178

ArrayExpress

E-GEOD-34178

BioProject

PRJNA149689

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Contact


Ahmed Aldarmahi