Computational protocol: VEZT, a Novel Putative Tumor Suppressor, Suppresses the Growth and Tumorigenicity of Gastric Cancer

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Protocol publication

[…] Purified total RNA was obtained from the microdissected cells. The whole human genome oligo microarray (Agilent, Santa Clara, CA, USA) was used. After hybridization and washing, the microarray slides were scanned with an Agilent DNA microarray scanner. The resulting text files extracted from Agilent Feature Extraction Software (version 9.5.3) were imported into the Agilent GeneSpring GX software (version 7.3) for further analysis. Differentially expressed genes were identified through fold-change screening. For target gene verification we used real-time PCR and chromatin immunoprecipitation assay. The primers for the target genes are listed in . […]

Pipeline specifications

Software tools Agilent Feature Extraction, GeneSpring GX
Application Gene expression microarray analysis
Organisms Homo sapiens, Helicobacter pylori
Diseases Neoplasms, Stomach Neoplasms