Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 14
Release date: Sep 1 2015
Last update date: Oct 24 2018
Access: Public
Diseases: Angelman Syndrome
Dataset link An Alternative Splicing Event Amplifies Evolutionary Differences Between Vertebrates

Experimental Protocol


This study contains two sets of samples: (Set 1) mRNA profiling of human 293 cells subjected to four different conditions in two biological replicates: non-targetting control siRNA, PTBP1 and PTBP2 siRNA, PTBP1 and PTBP2 siRNA with overexpression of full-length human PTBP1, PTBP1 and PTBP2 siRNA with overexpression of exon-excluded human PTBP1. (Set 2) mRNA profiling of chicken DT40 cells with 3 genotypes in two bioligcal replicates: wildtype cells, cells with PTBP1 exon 8 (orthologous to human PTBP1 exon 9) deleted in one allele, and cells with PTBP1 exon 8 deleted in both alleles.

Repositories


GEO

GSE69656

ArrayExpress

E-GEOD-69656

ENA

SRP059242

BioProject

PRJNA286111

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Contact


Serge Gueroussov