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Specifications


Application: RNA-seq analysis
Number of samples: 12
Release date: Aug 26 2014
Last update date: Aug 27 2014
Access: Public
Chemicals: Iron
Dataset link Transcriptome analysis of NTHi 86-028NPrpsL, NTHi 86-028NPrpsL∆fur, NTHi 86-028NPrpsL∆fur∆hrrF, NTHi 86-028NPrpsL∆fur∆hrrF3' strains

Experimental Protocol


These data comprise transcriptional anaylses of an rpsL mutant of 86-028NP, an isogenic fur mutant of 86-028NPrpsL, an isogenic fur mutant of 86-028NPrpsL also containing a full deletion of hrrF, and an isogenic fur mutant of 86-028NPrpsL also containing a deletion of only the 3' portion of hrrF. All strains were grown in defined medium containing 10 µg/ml human hemoglobin to mid-log phase. Cells were then harvested and RNA extracted. A total of three biological replicates were generated for these analyses. Analysis of transcriptomes using the Illumina HiSeq 2000 of four strains of nontypeable Haemophilus influenzae which include NTHi 86-028NPrpsL, NTHi 86-028NPrpsL∆fur, NTHi 86-028NPrpsL∆fur∆hrrF, and NTHi 86-028NPrpsL∆fur∆hrrF3' strains. For each strain three biological replicates were analyzed.

Repositories


GEO

GSE58891

ArrayExpress

E-GEOD-58891

ENA

SRP043631

BioProject

PRJNA253820

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