Self-organization of polarized cerebellar plate neuroepithelium in three-dimensional culture of human pluripotent stem cells

During cerebellar development, the main portion of the cerebellar plate neuroepithelium (NE) gives birth to Purkinje cells and interneurons, while the germinal zone at its dorsal edge, called the rhombic lip (RL), generates granule cells and cerebellar nuclei neurons. However, it remains elusive how these components work together to generate the intricate structure of the cerebellar anlage. In this study, we found that a polarized cerebellar anlage structure self-organizes in three-dimensional (3D) human ES cell (hESC) culture. This NE is capable of differentiating into electrophysiologically functional Purkinje cells. The addition of FGF19 promotes spontaneous generation of dorsoventrally polarized NE structures containing cerebellar and basal plates. Furthermore, further addition of SDF1 promoted the generation of stratified cerebellar plate NE with RL-like germinal zones self-forming at the edge. Thus, hESC-derived cerebellar progenitors exhibit substantial self-organizing potential for generating a polarized structure reminiscent of the early human cerebellar anlage at the first trimester.

Dataset features

Information

Specifications

Application:	RNA-seq analysis
Number of samples:	6
Release date:	Apr 15 2015
Last update date:	Oct 2 2018
Access:	Public
Diseases:	Cerebellar Diseases
Dataset link	Self-organization of polarized cerebellar plate neuroepithelium in three-dimensional culture of human pluripotent stem cells

Experimental Protocol

Examination of mRNA profile in two different treated human ES cells .

Repositories

GEO

ArrayExpress

ENA

BioProject

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Contact

Keiko Muguruma