Computational protocol: Structural visualization of key steps in human transcription initiation

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Protocol publication

[…] Data collection and image processing were conducted using the Leginon data collection software and the Appion electron microscopy processing environment, respectively. Three-dimensional maps were calculated using libraries from the EMAN2 and SPARX software packages,. Volume segmentation, automatic rigid body docking, generation were performed using UCSF Chimera. […]

Pipeline specifications

Software tools Leginon, Appion, EMAN, SPARX, UCSF Chimera
Application cryo-EM
Organisms Homo sapiens