Computational protocol: Effect of Blue Light on Endogenous Isopentenyladenine and Endoreduplication during Photomorphogenesis and De-Etiolation of Tomato (Solanum lycopersicum L.) Seedlings

Similar protocols

Protocol publication

[…] Germinated seeds were transferred to a new medium and Petri dishes were placed vertically in the culture chamber at 23°C. Seedlings grew for 5 days under continuous BL or in the dark (D). Hypocotyl length was measured with a ruler. Results represent averages of 10 seedlings ± SE.For the cell length measurement, the middle region of the hypocotyl of 5 days-old seedlings was excised and fixed for 30 min in 96% ethanol. Samples were immerged for 20 min in different gradient of ethanol solution (80%, 70% and 50%), washed 2 times in water for 10 min and finally stained for 10 min in a solution of toluidine blue (1%, w/v). Fragments were mounted in a drop of water and pictures were taken with a microscope (Olympus BX60, Japan) equipped with a camera (Olympus DP71, Japan). Cell length was measured using the ImageJ software (a public domain, Java-based image processing program developed at the National Institutes of Health; For each genotype and light conditions, cells of 5 hypocotyls were monitored. [...] The statistical significance of the treatment differences was assessed by the one- or two-way ANOVA followed by the Bonferroni post-hoc test or by the t-test of Student performed with the Statistica software version 6 (Stat Soft, Inc.). The tool allowing the description of correlation was also used. […]

Pipeline specifications

Software tools ImageJ, Statistica
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Solanum lycopersicum, Arabidopsis thaliana