Computational protocol: Human Metapneumovirus: Insights from a Ten-Year Molecular and Epidemiological Analysis in Germany

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Protocol publication

[…] A multiple sequence alignment was compiled from a part (473 nt) of the FI subunit of the F gene using ClustalW in the Bioedit version 7.0.9 . Maximum likelihood tree was constructed by using Geneious 5.0.4 . The reliability of the branching order was estimated by performing 1,000 bootstrap replicates with the HKY85 model. Phylogenetic analysis included representative sequences of the described HMPV lineages and subgroups. GenBank accession numbers and the country of isolation of the reference sequences are given in the supporting information (). The tree was manually edited in Corel Draw 12 program.Pairwise nucleotide and amino acid distances within and between groups were calculated using MEGA and determined distances described in terms of mean and standard deviation. […]

Pipeline specifications

Software tools Clustal W, BioEdit, Geneious, MEGA
Application Phylogenetics
Organisms Human metapneumovirus, Homo sapiens
Diseases Bronchitis, Infection, Influenza, Human, Pneumonia, Respiratory Insufficiency