Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Mar 19 2012
Last update date: Sep 3 2014
Access: Public
Diseases: Pleuropneumonia
Chemicals: Catecholamines, Epinephrine, Norepinephrine
Dataset link Expression data of Actinobacillus pleuropneumoniae 4074 in response to epinephrine and norepinephrine

Experimental Protocol


Transcriptional profiles were analyzed using microarray to compared the epinephrine (Epi) or norepinephrine(NE) treated and untreated A. pleuropneumoniae. All samples were harvested from middle exponential phase (4 hours after sub-culture) at the OD600=1.435±0.065 for control, OD600=1.461±0.019 for Epi treated strain and OD600=1.545±0.115 for NE treated strain. Three biological replicates were conducted for each treatment. The total RNA were extracted and hybridized with the whole genome microarray of A. pleuropneumoniae. The signal intensities were normalized and transformed into log2 values. The genes with fold change ≥ 1.5 and P < 0.05 were selected as differentially expressed.

Repositories


GEO

GSE25516

ArrayExpress

E-GEOD-25516

BioProject

PRJNA133769

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Contact


Lu Li
Zhuofei Xu
Rui Zhou
Huanchun Chen

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