|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Jan 30 2015|
|Last update date:||Aug 9 2018|
|Dataset link||Functional and evolutionary significance of human microRNA seed region mutations [M12]|
mRNAs were collected from 3 M12 miRNA treated and 3 negative control miRNA treated HEY ovarian cancer cell samples. mRNA expression was captured on Affymetrix U133 Plus 2 chips. To compare mRNA expression pattern between the M12 treated cells and the negative control treated cells, present/absent calls were generated using MAS5, while signals were calculated using GCRMA and then log2 transformed. Expression of differentially expressed genes or down regulated miRanda-mirSVR predicted miRNA target genes was compared between miRNA treated samples.
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