Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jan 30 2015
Last update date: Aug 9 2018
Access: Public
Diseases: Neoplasms
Chemicals: Nucleotides
Dataset link Functional and evolutionary significance of human microRNA seed region mutations [M12]

Experimental Protocol


mRNAs were collected from 3 M12 miRNA treated and 3 negative control miRNA treated HEY ovarian cancer cell samples. mRNA expression was captured on Affymetrix U133 Plus 2 chips. To compare mRNA expression pattern between the M12 treated cells and the negative control treated cells, present/absent calls were generated using MAS5, while signals were calculated using GCRMA and then log2 transformed. Expression of differentially expressed genes or down regulated miRanda-mirSVR predicted miRNA target genes was compared between miRNA treated samples.

Repositories


GEO

GSE56972

ArrayExpress

E-GEOD-56972

BioProject

PRJNA245097

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Contact


Neda Jabbari

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