Computational protocol: The Epitope of Monoclonal Antibodies Blocking Erythrocyte Invasion by Plasmodium falciparum Map to The Dimerization and Receptor Glycan Binding Sites of EBA-175

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Protocol publication

[…] PepSurf and Mapitope were used to map the peptides found in the phage display libraries for mAb R215, R217, and R256 to the crystal structure of EBA-175 RII (PDB: 1ZRO) . The PepSurf and Mapitope results for the R215, R217, and R256 mAbs were cross-referenced with each other and those positions identified for all three mAbs were defined as putative epitope residues. These residues were used to guide a visual analysis of the crystal structure to select the peptide fragment for experimental characterization. Solvent accessible surface area calculations for the predicted epitopes were performed with AREAIMOL of CCP4 . […]

Pipeline specifications

Software tools PepSurf, Mapitope
Application Immune system analysis
Organisms Plasmodium falciparum, Homo sapiens
Diseases Malaria
Chemicals Glycophorin, N-Acetylneuraminic Acid